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验证数据展示

  • IF Staining of HeLa using CL594-67314

    IF Staining of HeLa using CL594-67314

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated TRIM25 antibody (CL594-67314, Clone: 1H6G1 ) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated TRIM25 Monoclonal antibody

TRIM25 Monoclonal Antibody for IF/ICC
Cat No. CL594-67314

产品说明书

CloneNo. 1H6G1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC

别名

EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TRIM25 Ready-To-Use IHC Kit. TRIM25 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67314-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67314 targets TRIM25 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 TRIM25 fusion protein Ag29325 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tripartite motif-containing 25
别名 EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147
计算分子量 630 aa, 71 kDa
观测分子量 68-71 kDa
GenBank蛋白编号BC016924
基因名称 TRIM25
Gene ID (NCBI) 7706
RRIDAB_2920111
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14258
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).

实验方案

Product Specific Protocols
IF protocol for CL594 TRIM25 antibody CL594-67314Download protocol
Standard Protocols
Click here to view our Standard Protocols