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验证数据展示

  • IF Staining of HeLa using CL594-67314

    IF Staining of HeLa using CL594-67314

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated TRIM25 antibody (CL594-67314, Clone: 1H6G1 ) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated TRIM25 Monoclonal antibody

TRIM25 Monoclonal Antibody for IF/ICC
Cat No. CL594-67314

Print datasheet

CloneNo. 1H6G1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TRIM25 Ready-To-Use IHC Kit. TRIM25 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67314-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67314 targets TRIM25 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen TRIM25 fusion protein Ag29325 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tripartite motif-containing 25
Synonyms EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight 68-71 kDa
GenBank Accession NumberBC016924
Gene Symbol TRIM25
Gene ID (NCBI) 7706
RRIDAB_2920111
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14258
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).

实验方案

Product Specific Protocols
IF protocol for CL594 TRIM25 antibody CL594-67314Download protocol
Standard Protocols
Click here to view our Standard Protocols