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验证数据展示

  • WB analysis using 84661-4-RR

    WB analysis using 84661-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84661-4-RR (TRIM26 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84661-4-RR

    Affinity and Kinetic Characterization of 84661-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84661-4-RR against Human TRIM26 were performed. The affinity constant is 47.3 pM.

TRIM26 Recombinant antibody

TRIM26 Recombinant Antibody for WB, ELISA
Cat No. 84661-4-RR

产品说明书

CloneNo. 242154B5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

ZNF173, Tripartite motif-containing protein 26, TRIM, RNF95, EC:2.3.2.27

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy TRIM26 Ready-To-Use IHC Kit. TRIM26 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84661-4-RR targets TRIM26 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 TRIM26 fusion protein Ag25546 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 26
别名 ZNF173, Tripartite motif-containing protein 26, TRIM, RNF95, EC:2.3.2.27
计算分子量 62 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC024039
基因名称 TRIM26
Gene ID (NCBI) 7726
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ12899
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM26 is a member of the tripartite motif (TRIM) protein family composed of more than 70 members in human. TRIM proteins share a similar characteristic structure, which includes a RING (R) domain, one or two B-boxes (B), and a coiled coil (CC) domain in the N-terminal and a domain in the C-terminal with variable structures. TRIM26 potentiates virus-triggered IRF3, NF-κB activation, IFN-β induction, and cellular antiviral responses. Further investigation suggests that TRIM26 plays an important role in the antiviral innate immune response by bridging TBK1 to NEMO and mediating TBK1 activation. (PMID: 25763818, PMID: 26611359)

实验方案

Product Specific Protocols
WB protocol for TRIM26 antibody 84661-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols