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验证数据展示

  • IF Staining of HepG2 using 84927-6-RR

    IF Staining of HepG2 using 84927-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM27 antibody (84927-6-RR, Clone: 242538H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 84927-6-RR

    IF Staining of HeLa using 84927-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM27 antibody (84927-6-RR, Clone: 242538H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 84927-6-RR

    FC experiment of HepG2 using 84927-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Trim27 Recombinant Antibody (84927-6-RR, Clone:242538H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HeLa using 84927-6-RR

    FC experiment of HeLa using 84927-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Trim27 Recombinant Antibody (84927-6-RR, Clone:242538H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84927-6-RR

    Affinity and Kinetic Characterization of 84927-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84927-6-RR against Human TRIM27 were performed. The affinity constant is 0.653 nM.

TRIM27 Recombinant antibody

TRIM27 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84927-6-RR

产品说明书

CloneNo. 242538H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Tripartite motif-containing protein 27, TRIM, RNF76, RFP, EC:2.3.2.27

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy TRIM27 Ready-To-Use IHC Kit. TRIM27 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84927-6-RR targets TRIM27 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 TRIM27 fusion protein Ag2849 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 27
别名 Tripartite motif-containing protein 27, TRIM, RNF76, RFP, EC:2.3.2.27
计算分子量 513 aa, 58 kDa
GenBank蛋白编号BC013580
基因名称 TRIM27
Gene ID (NCBI) 5987
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP14373
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TRIM27 antibody 84927-6-RRDownload protocol
FC protocol for TRIM27 antibody 84927-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols