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验证数据展示

  • WB analysis using 83175-2-RR (same clone as 83175-2-PBS)

    WB analysis using 83175-2-RR (same clone as 83175-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83175-2-RR (TRIM54 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83175-2-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 83175-2-RR (same clone as 83175-2-PBS)

    IF Staining of C2C12 using 83175-2-RR (same clone as 83175-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using TRIM54 antibody (83175-2-RR, Clone: 230391G5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83175-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83175-2-RR (same clone as 83175-2-PBS)

    IF Staining of HepG2 using 83175-2-RR (same clone as 83175-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM54 antibody (83175-2-RR, Clone: 230391G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83175-2-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83175-2-RR (same clone as 83175-2-PBS)

    FC experiment of MCF-7 using 83175-2-RR (same clone as 83175-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Trim54 Recombinant Antibody (83175-2-RR, Clone:230391G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83175-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00236-3

    Cytometric bead array standard curve of MP00236-3

    Cytometric bead array standard curve of MP00236-3, TRIM54 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83175-1-PBS. Detection antibody: 83175-2-PBS. Standard: Ag15229. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83175-2-RR

    Affinity and Kinetic Characterization of 83175-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83175-2-RR against Human TRIM54 were performed. The affinity constant is below 1 pM.

TRIM54 Recombinant antibody, PBS Only (Detector)

TRIM54 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83175-2-PBS

产品说明书

CloneNo. 230391G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

RNF30, Muscle-specific RING finger protein 3, Muscle-specific RING finger protein, MuRF-3, MuRF3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83175-2-PBS targets TRIM54 as part of a matched antibody pair:

MP00236-3: 83175-1-PBS capture and 83175-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TRIM54 fusion protein Ag15229 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 54
别名 RNF30, Muscle-specific RING finger protein 3, Muscle-specific RING finger protein, MuRF-3, MuRF3
计算分子量 358 aa, 40 kDa
观测分子量40 kDa
GenBank蛋白编号BC141807
基因名称 TRIM54
Gene ID (NCBI) 57159
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BYV2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM54 (Tripartite motif-containing protein 54) is also names as MURF, MURF3, RNF30. The expression of TRIM54 is required for skeletal myoblast differentiation and myotube fusion. It is expressed in heart and skeletal muscle specifically (PMID:11243782). It has two isoforms with the molecular weight of 40KDa and 45KDa. It can be dimerizated and hetero-dimerizated to form homooligomer and heterooligomer (PMID:15967462).