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验证数据展示

  • WB analysis using 83175-2-RR

    WB analysis using 83175-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83175-2-RR (TRIM54 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • IF Staining of C2C12 using 83175-2-RR

    IF Staining of C2C12 using 83175-2-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using TRIM54 antibody (83175-2-RR, Clone: 230391G5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HepG2 using 83175-2-RR

    IF Staining of HepG2 using 83175-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM54 antibody (83175-2-RR, Clone: 230391G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of MCF-7 using 83175-2-RR

    FC experiment of MCF-7 using 83175-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Trim54 Recombinant Antibody (83175-2-RR, Clone:230391G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83175-2-RR

    Affinity and Kinetic Characterization of 83175-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83175-2-RR against Human TRIM54 were performed. The affinity constant is below 1 pM.

TRIM54 Recombinant antibody

TRIM54 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83175-2-RR

产品说明书

CloneNo. 230391G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

RNF30, Muscle-specific RING finger protein 3, Muscle-specific RING finger protein, MuRF-3, MuRF3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, mouse heart tissue
Positive IF/ICC detected inC2C12 cells, HepG2 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:14000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83175-2-RR targets TRIM54 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 TRIM54 fusion protein Ag15229 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 54
别名 RNF30, Muscle-specific RING finger protein 3, Muscle-specific RING finger protein, MuRF-3, MuRF3
计算分子量 358 aa, 40 kDa
观测分子量40 kDa
GenBank蛋白编号BC141807
基因名称 TRIM54
Gene ID (NCBI) 57159
RRIDAB_3670869
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BYV2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM54 (Tripartite motif-containing protein 54) is also names as MURF, MURF3, RNF30. The expression of TRIM54 is required for skeletal myoblast differentiation and myotube fusion. It is expressed in heart and skeletal muscle specifically (PMID:11243782). It has two isoforms with the molecular weight of 40KDa and 45KDa. It can be dimerizated and hetero-dimerizated to form homooligomer and heterooligomer (PMID:15967462).

实验方案

Product Specific Protocols
WB protocol for TRIM54 antibody 83175-2-RRDownload protocol
IF protocol for TRIM54 antibody 83175-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols