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TRIM72 Monoclonal antibody

TRIM72 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5F2C11

Cat no : 67403-1-Ig

Print datasheet

Synonyms

tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53

验证数据展示

  • FC experiment of C2C12 using 67403-1-Ig

    FC experiment of C2C12 using 67403-1-Ig

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human TRIM72 (67403-1-Ig, Clone:5F2C11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 67403-1-Ig

    WB analysis using 67403-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skeletal muscle using 67403-1-Ig

    IF Staining of mouse skeletal muscle using 67403-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse skeletal muscle using 67403-1-Ig

    IF Staining of mouse skeletal muscle using 67403-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHCT 116 cells, pig heart tissue, mouse skeletal muscle tissue, mouse heart tissue, pig skeletal muscle tissue
Positive IF-P detected inmouse skeletal muscle tissue
Positive FC (Intra) detected inC2C12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67403-1-Ig targets TRIM72 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen TRIM72 fusion protein Ag2560 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tripartite motif-containing 72
Synonyms tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC033211
Gene Symbol TRIM72
Gene ID (NCBI) 493829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6ZMU5
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRIM72 antibody 67403-1-IgDownload protocol
IF protocol for TRIM72 antibody 67403-1-IgDownload protocol
FC protocol for TRIM72 antibody 67403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols