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验证数据展示

  • FC experiment of C2C12 using 67403-1-Ig

    FC experiment of C2C12 using 67403-1-Ig

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human TRIM72 (67403-1-Ig, Clone:5F2C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 67403-1-Ig

    WB analysis using 67403-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skeletal muscle using 67403-1-Ig

    IF Staining of mouse skeletal muscle using 67403-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRIM72 Monoclonal antibody

TRIM72 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA
Cat No. 67403-1-Ig

Print datasheet

CloneNo. 5F2C11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF-P, FC (Intra), ELISA

Synonyms

tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, pig heart tissue, mouse skeletal muscle tissue, mouse heart tissue, pig skeletal muscle tissue
Positive IF-P detected inmouse skeletal muscle tissue
Positive FC (Intra) detected inC2C12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67403-1-Ig targets TRIM72 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen TRIM72 fusion protein Ag2560 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tripartite motif-containing 72
Synonyms tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC033211
Gene Symbol TRIM72
Gene ID (NCBI) 493829
RRIDAB_3085040
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6ZMU5
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRIM72 antibody 67403-1-IgDownload protocol
IF protocol for TRIM72 antibody 67403-1-IgDownload protocol
FC protocol for TRIM72 antibody 67403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols