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TRIM72 Monoclonal antibody, PBS Only

TRIM72 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5F2C11

Cat no : 67403-1-PBS

Print datasheet

Synonyms

tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53

验证数据展示

  • FC experiment of C2C12 using 67403-1-Ig (same clone as 67403-1-PBS)

    FC experiment of C2C12 using 67403-1-Ig (same clone as 67403-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human TRIM72 (67403-1-Ig, Clone:5F2C11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

  • WB analysis using 67403-1-Ig (same clone as 67403-1-PBS)

    WB analysis using 67403-1-Ig (same clone as 67403-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

    IF Staining of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

    IF Staining of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

产品信息

67403-1-PBS targets TRIM72 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen TRIM72 fusion protein Ag2560 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tripartite motif-containing 72
Synonyms tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC033211
Gene Symbol TRIM72
Gene ID (NCBI) 493829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6ZMU5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C