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验证数据展示

  • IF Staining of U2OS using CL488-29087

    IF Staining of U2OS using CL488-29087

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 TRMT10C antibody (CL488-29087) at dilution of 1:200.

CoraLite® Plus 488-conjugated TRMT10C Polyclonal antibody

TRMT10C Polyclonal Antibody for IF/ICC
Cat No. CL488-29087

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

RG9MTD1, MRPP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy TRMT10C Ready-To-Use IHC Kit. TRMT10C primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 29087-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-29087 targets TRMT10C in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 TRMT10C fusion protein Ag30642 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA (guanine-9-) methyltransferase domain containing 1
别名 RG9MTD1, MRPP1
计算分子量 47 kDa
观测分子量40-45 kDa
GenBank蛋白编号NM_017819
基因名称 TRMT10C
Gene ID (NCBI) 54931
RRIDAB_3672836
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L0Y3
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

tRNA methyltransferase 10 homolog C (TRMT10C, also known as MRPP1) is involved in mitochondrial RNA processing and modification (PMID: 21593607). It is essential for the 5'-ends processing of mitochondrial tRNAs and plays a critical role in mitochondrial respiration and translation (PMID: 18984158; 29040705). Mutations in TRMT10C may be a cause of mitochondrial disease and highlight the importance of RNA processing for correct mitochondrial function (PMID: 27132592).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRMT10C antibody CL488-29087Download protocol
Standard Protocols
Click here to view our Standard Protocols