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TRNP1 Recombinant antibody

TRNP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240331C6

Cat no : 83410-3-RR

Print datasheet

Synonyms

TRNP, TNRP, TMF-regulated nuclear protein 1, C1orf225, 240331C6

验证数据展示

  • WB analysis using 83410-3-RR

    WB analysis using 83410-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83410-3-RR (TRNP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83410-3-RR

    FC experiment of HeLa using 83410-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRNP1 Recombinant antibody (83410-3-RR, Clone:240331C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83410-3-RR

    Affinity and Kinetic Characterization of 83410-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83410-3-RR against Human TRNP1 were performed. The affinity constant is 72.8 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, SH-SY5Y cells, rat heart tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83410-3-RR targets TRNP1 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen TRNP1 fusion protein Ag22504 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TMF1-regulated nuclear protein 1
Synonyms TRNP, TNRP, TMF-regulated nuclear protein 1, C1orf225, 240331C6
Calculated Molecular Weight 227 aa, 23 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberBC069216
Gene Symbol TRNP1
Gene ID (NCBI) 388610
RRIDAB_3671057
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6NT89
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRNP1 (TMF-regulated nuclear protein 1), also called C1orf225, is a DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex (PMID: 27264089; 23622239; 36947129). It has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding (PMID: 32627867). It may also control the proliferation rate of cells by regulating their progression through key cell-cycle transition points.

实验方案

Product Specific Protocols
WB protocol for TRNP1 antibody 83410-3-RRDownload protocol
FC protocol for TRNP1 antibody 83410-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols