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  • Featured Product
  • KD/KO Validated

TRPC6 Polyclonal antibody

TRPC6 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18236-1-AP

Print datasheet

Synonyms

TRPC 6, TRP-6, TRP6, TRP 6, Transient receptor protein 6

验证数据展示

  • WB analysis of mouse brain using 18236-1-AP

    WB analysis of mouse brain using 18236-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500.

  • IHC staining of human placenta using 18236-1-AP

    IHC staining of human placenta using 18236-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 18236-1-AP

    IHC staining of human placenta using 18236-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse lung using 18236-1-AP

    IP experiment of mouse lung using 18236-1-AP

    IP result of anti-TRPC6 (IP:18236-1-AP, 4ug; Detection:18236-1-AP 1:300) with mouse lung tissue lysate 3600ug.

  • FC experiment of A549 using 18236-1-AP

    FC experiment of A549 using 18236-1-AP

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human TRPC6 (18236-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of human placenta using 18236-1-AP

    IF Staining of human placenta using 18236-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using TRPC6 antibody (18236-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 18236-1-AP

    IF Staining of A549 using 18236-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRPC6 antibody (18236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain, COLO 320 cells, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18236-1-AP targets TRPC6 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen TRPC6 fusion protein Ag12976 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily C, member 6
Synonyms TRPC 6, TRP-6, TRP6, TRP 6, Transient receptor protein 6
Calculated Molecular Weight 931 aa, 106 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC093658
Gene Symbol TRPC6
Gene ID (NCBI) 7225
RRIDAB_10859822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y210
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).

实验方案

Product Specific Protocols
WB protocol for TRPC6 antibody 18236-1-APDownload protocol
IHC protocol for TRPC6 antibody 18236-1-APDownload protocol
IF protocol for TRPC6 antibody 18236-1-APDownload protocol
IP protocol for TRPC6 antibody 18236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanFC

Cell

Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis.

Authors - Wei Rao
View Article
mouseWB,IF

Biomaterials

Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.

Authors - Meitian Wang
View Article
humanIF

Proc Natl Acad Sci U S A

TRPC6 channel translocation into phagosomal membrane augments phagosomal function.

Authors - Vladimir Riazanski
  • KO Validated
View Article
mouseWB

Phytomedicine

Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro

Authors - Zhongzhu Ai
View Article
mouseWB

Br J Pharmacol

Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro.

Authors - Jiangnan Zhang
View Article
ratWB

Lab Invest

Amelioration of circadian disruption and calcium-handling protein defects by choline alleviates cardiac remodeling in abdominal aorta coarctation rats.

Authors - Xi He
View Article