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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TRPC6 Polyclonal antibody

TRPC6 Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18236

Print datasheet

Synonyms

TRPC 6, TRP-6, TRP6, TRP 6, Transient receptor protein 6

验证数据展示

  • FC experiment of A549 using CL488-18236

    FC experiment of A549 using CL488-18236

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated TRPC6 Polyclonal antibody (CL488-18236)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of human placenta using CL488-18236

    IF Staining of human placenta using CL488-18236

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 TRPC6 antibody (CL488-18236) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inhuman placenta tissue
Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 18236-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18236 targets TRPC6 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen TRPC6 fusion protein Ag12976 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily C, member 6
Synonyms TRPC 6, TRP-6, TRP6, TRP 6, Transient receptor protein 6
Calculated Molecular Weight 931 aa, 106 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC093658
Gene Symbol TRPC6
Gene ID (NCBI) 7225
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y210
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRPC6 antibody CL488-18236Download protocol
FC protocol for CL Plus 488 TRPC6 antibody CL488-18236Download protocol
Standard Protocols
Click here to view our Standard Protocols