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TRPS1 Recombinant antibody

TRPS1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230051F10

Cat no : 83062-4-RR

Print datasheet

Synonyms

Tricho-rhino-phalangeal syndrome type I protein, LGCR, GC79, 230051F10

验证数据展示

  • WB analysis using 83062-4-RR

    WB analysis using 83062-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83062-4-RR (TRPS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 83062-4-RR

    FC experiment of MCF-7 using 83062-4-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TRPS1 Recombinant antibody (83062-4-RR, Clone:230051F10)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of human breast cancer using 83062-4-RR

    IHC staining of human breast cancer using 83062-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 83062-4-RR (TRPS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83062-4-RR

    Affinity and Kinetic Characterization of 83062-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83062-4-RR against Human TRPS1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83062-4-RR targets TRPS1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen TRPS1 fusion protein Ag33959 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name trichorhinophalangeal syndrome I
Synonyms Tricho-rhino-phalangeal syndrome type I protein, LGCR, GC79, 230051F10
Calculated Molecular Weight 1294 aa, 143 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC125020
Gene Symbol TRPS1
Gene ID (NCBI) 7227
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains.

实验方案

Product Specific Protocols
WB protocol for TRPS1 antibody 83062-4-RRDownload protocol
IHC protocol for TRPS1 antibody 83062-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols