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TRPV2 Recombinant antibody

TRPV2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230141C3

Cat no : 83022-1-RR

Print datasheet

Synonyms

VRL, Vanilloid receptor-like protein 1, OTRPC2, Osm-9-like TRP channel 2, Osm 9 like TRP channel 2

验证数据展示

  • WB analysis of HT-1080 using 83022-1-RR

    WB analysis of HT-1080 using 83022-1-RR

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 83022-1-RR (TRPV2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of K562 using 83022-1-RR

    FC experiment of K562 using 83022-1-RR

    1x10^6 K562 were intracellularly stained with 0.2 ug TRPV2 Recombinant antibody (83022-1-RR, Clone:230141C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83022-1-RR

    Affinity and Kinetic Characterization of 83022-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83022-1-RR against Human TRPV2 were performed. The affinity constant is 31.3 pM.

经过测试的应用

Positive WB detected inHT-1080 cells
Positive FC (Intra) detected inK562

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83022-1-RR targets TRPV2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen TRPV2 fusion protein Ag8864 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transient receptor potential cation channel, subfamily V, member 2
Synonyms VRL, Vanilloid receptor-like protein 1, OTRPC2, Osm-9-like TRP channel 2, Osm 9 like TRP channel 2
Calculated Molecular Weight 764 aa, 86 kDa
Observed Molecular Weight~80 kDa
GenBank Accession NumberBC018926
Gene Symbol TRPV2
Gene ID (NCBI) 51393
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5S1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transient receptor potential vanilloid 2 (TRPV2), also name as VRL-1, plays a critical role in neuronal development, cardiac function, immunity, and cancer (PMID: 31566564). It has been found to play an essential role in sustained intracellular Ca2+ concentration increase (PMID: 31857697). It's reported that TRPV2 is activated by high temperatures, with a threshold of around 52℃ (PMID: 10201375).

实验方案

Product Specific Protocols
WB protocol for TRPV2 antibody 83022-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols