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TRPV4 Polyclonal antibody

TRPV4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27892-1-AP

Print datasheet

Synonyms

Osm 9 like TRP channel 4, OTRPC4, TRP12, TRPV4, VR OAC, VRL 2, VRL2, VROAC

验证数据展示

  • WB analysis using 27892-1-AP

    WB analysis using 27892-1-AP

    SH-SY5Y cell lysates (heated at 100℃, heated at 37℃) were subjected to SDS PAGE followed by western blot with 27892-1-AP (TRPV4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected in37°C incubated SH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27892-1-AP targets TRPV4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen TRPV4 fusion protein Ag27253 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily V, member 4
Synonyms Osm 9 like TRP channel 4, OTRPC4, TRP12, TRPV4, VR OAC, VRL 2, VRL2, VROAC
Calculated Molecular Weight 98 kDa
Observed Molecular Weight 85~98 kDa
GenBank Accession NumberNM_001177428.1
Gene Symbol TRPV4
Gene ID (NCBI) 59341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBA0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPV4, also named as VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.

实验方案

Product Specific Protocols
WB protocol for TRPV4 antibody 27892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols