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TRUB1 Polyclonal antibody

TRUB1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12520-1-AP

Print datasheet

Synonyms

PUS4, TRUB1

验证数据展示

  • WB analysis using 12520-1-AP

    WB analysis using 12520-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12520-1-AP (TRUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 12520-1-AP

    IP experiment of mouse liver using 12520-1-AP

    IP result of anti-TRUB1 (IP:12520-1-AP, 4ug; Detection:12520-1-AP 1:300) with mouse liver tissue lysate 6400ug.

  • IHC staining of human kidney using 12520-1-AP

    IHC staining of human kidney using 12520-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12520-1-AP (TRUB1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 12520-1-AP

    IHC staining of human kidney using 12520-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12520-1-AP (TRUB1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse testis using 12520-1-AP

    IHC staining of mouse testis using 12520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12520-1-AP (TRUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 12520-1-AP

    IHC staining of mouse testis using 12520-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12520-1-AP (TRUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12520-1-AP targets TRUB1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TRUB1 fusion protein Ag3213 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TruB pseudouridine (psi) synthase homolog 1 (E. coli)
Synonyms PUS4, TRUB1
Calculated Molecular Weight 349 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC030601
Gene Symbol TRUB1
Gene ID (NCBI) 142940
RRIDAB_10640437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWH5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRUB1 gene encodes a 349-amino acid product, with a VFAVHKPKGPTSA box in positions 71-83 corresponding to motif I of the TruB family (probably involved in conserving protein structure). This protein may be responsible for synthesis of pseudouridine from uracil in transfer RNAs.

实验方案

Product Specific Protocols
WB protocol for TRUB1 antibody 12520-1-APDownload protocol
IHC protocol for TRUB1 antibody 12520-1-APDownload protocol
IP protocol for TRUB1 antibody 12520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Methods

Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome

Authors - Haiqi Xu
View Article
humanIF

bioRxiv

Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess neuronal epitranscriptome plasticity

Authors - Oleksandra Fanari
View Article
humanWB

Nucleic Acids Res

A bisulfite-assisted and ligation-based qPCR amplification technology for locus-specific pseudouridine detection at base resolution

Authors - Xin Fang
  • KD Validated
View Article