验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, HEK-293 cells, human testis tissue, MCF-7 cells, mouse testis tissue |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 3 publications below |
产品信息
12574-1-AP targets TSP50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | TSP50 fusion protein Ag3247 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | testes-specific protease 50 |
| 别名 | testes protease 50, Serine protease 50, PRSS50, EC:3.4.25.-, CT20 |
| 计算分子量 | 385 aa, 43 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC033016 |
| 基因名称 | TSP50 |
| Gene ID (NCBI) | 29122 |
| RRID | AB_10646475 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UI38 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSP50 (Testis-specific protease-like protein 50) is also named as PRSS50, CT20 and belongs to the peptidase S1 family. It can enhance the expression of NFKB target genes involved in cell proliferation, and promotes cell proliferation, at least partially, through activation of the NFKB signalling pathway(PMID:21385156). There are two protein bands of 37 kDa and 43 kDa to be detected in human testes that the the difference is most likely caused by posttranslational proteolytic cleavage(PMID:11782390). The full length protein has a signal peptide with 39 amino acids and two glycosylation sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TSP50 antibody 12574-1-AP | Download protocol |
| IHC protocol for TSP50 antibody 12574-1-AP | Download protocol |
| IF protocol for TSP50 antibody 12574-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Cancer Testes-specific protease 50 (TSP50) promotes invasion and metastasis by inducing EMT in gastric cancer.
| ||
Oncol Rep Lentivirus-mediated knockdown of TSP50 suppresses the growth of non-small cell lung cancer cells via G0/G1 phase arrest. | ||
Gastroenterol Res Pract Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer. | ||
Oncol Lett Testes-specific protease 50 as an independent risk factor for poor prognosis in patients with non-small cell lung cancer. |
