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TTC26 Polyclonal antibody

TTC26 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25083-1-AP

Print datasheet

Synonyms

IFT56, dyf 13

验证数据展示

  • WB analysis of mouse testis using 25083-1-AP

    WB analysis of mouse testis using 25083-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25083-1-AP (TTC26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 25083-1-AP

    IF Staining of hTERT-RPE1 using 25083-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using TTC26 antibody (25083-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CoraLite®594 ARL13B antibody (CL594-17711, red).

经过测试的应用

Positive WB detected inmouse testis tissue, HEK-293 cells, NIH/3T3 cells
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25083-1-AP targets TTC26 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TTC26 fusion protein Ag18983 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetratricopeptide repeat domain 26
Synonyms IFT56, dyf 13
Calculated Molecular Weight 554 aa, 64 kDa
Observed Molecular Weight 60-64 kDa
GenBank Accession NumberBC126331
Gene Symbol TTC26
Gene ID (NCBI) 79989
RRIDAB_2651173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA0AVF1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTC26 (Tetratricopeptide repeat protein 26), also known as IFT56 (Intraflagellar transport protein 56), is an IFT complex B protein, which has a role in transport of ciliary cargo, important for ciliary motility and construction of full length cilia (PMID: 38135897). Severe biliary ciliopathy, caused by bi-allelic mutations in TTC26, has been recently described in the context of a syndrome of polydactyly and severe neonatal cholestasis, with brain, kidney and heart involvement (PMID: 32617964).

实验方案

Product Specific Protocols
WB protocol for TTC26 antibody 25083-1-APDownload protocol
IF protocol for TTC26 antibody 25083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Authors - Tomoharu Kanie
View Article