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  • Featured Product
  • KD/KO Validated

TTC30A Polyclonal antibody

TTC30A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25352-1-AP

Print datasheet

Synonyms

IFT70A, TPR repeat protein 30A, Intraflagellar transport protein 70A

验证数据展示

  • WB analysis using 25352-1-AP

    WB analysis using 25352-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25352-1-AP (TTC30A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 25352-1-AP

    WB analysis of A431 using 25352-1-AP

    WB result of TTC30A antibody (25352-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC30A transfected A431 cells.

  • IF Staining of hTERT-RPE1 using 25352-1-AP

    IF Staining of hTERT-RPE1 using 25352-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC30A antibody (25352-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • IP experiment of HEK-293 using 25352-1-AP

    IP experiment of HEK-293 using 25352-1-AP

    IP result of anti-TTC30A (IP:25352-1-AP, 4ug; Detection:25352-1-AP 1:500) with HEK-293 cells lysate 2600ug.

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, mouse testis, rat testis
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25352-1-AP targets TTC30A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TTC30A fusion protein Ag18361 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetratricopeptide repeat domain 30A
Synonyms IFT70A, TPR repeat protein 30A, Intraflagellar transport protein 70A
Calculated Molecular Weight 665 aa, 76 kDa
Observed Molecular Weight 72-76 kDa
GenBank Accession NumberBC042848
Gene Symbol TTC30A
Gene ID (NCBI) 92104
RRIDAB_2651172
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TTC30A antibody 25352-1-APDownload protocol
IF protocol for TTC30A antibody 25352-1-APDownload protocol
IP protocol for TTC30A antibody 25352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Authors - Tomoharu Kanie
View Article
humanWB,IF

Biol Open

Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.

Authors - Ryota Takei
View Article