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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TTC30A Polyclonal antibody

TTC30A Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25352

Print datasheet

Synonyms

IFT70, TPR repeat protein 30A, TTC30A

验证数据展示

  • IF Staining of hTERT-RPE1 using CL488-25352

    IF Staining of hTERT-RPE1 using CL488-25352

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 TTC30A antibody (CL488-25352) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 25352-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25352 targets TTC30A in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen TTC30A fusion protein Ag18361 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetratricopeptide repeat domain 30A
Synonyms IFT70, TPR repeat protein 30A, TTC30A
Calculated Molecular Weight 665 aa, 76 kDa
Observed Molecular Weight 72-76 kDa
GenBank Accession NumberBC042848
Gene Symbol TTC30A
Gene ID (NCBI) 92104
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WT1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tetratricopeptide repeat protein 30A (TTC30A) as a component of ciliary segmentation, may be essential for cartilage differentiation and renal tubulogenesis (PMID: 34548398). It is required for polyglutamylation of axonemal tubulin and plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TTC30A antibody CL488-25352Download protocol
Standard Protocols
Click here to view our Standard Protocols