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验证数据展示

  • WB analysis using 84744-1-RR

    WB analysis using 84744-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 84744-1-RR (TTK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

TTK Recombinant antibody

TTK Recombinant Antibody for WB, ELISA
Cat No. 84744-1-RR

Print datasheet

CloneNo. 241873B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, DU 145 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy TTK Ready-To-Use IHC Kit. TTK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84744-1-RR targets TTK in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TTK fusion protein Ag20556 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TTK protein kinase
Synonyms
Calculated Molecular Weight 97 kDa
Observed Molecular Weight97 kDa
GenBank Accession NumberBC000633
Gene Symbol TTK
Gene ID (NCBI) 7272
RRIDAB_3672129
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP33981
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mps1 family of kinases colocalizes with mitotic checkpoint proteins in kinetochores and/or spindle pole bodies/centrosomes. TTK has been shown to be a cell cycle-regulated kinase displaying maximum activity during M phase(PMID:15618221). Its kinase activity is required for proper chromosome segregation during mitosis through its involvements in microtubule-chromosome attachment error correction and the mitotic checkpoint(PMID:20937696). TTK mRNA is present at relatively high levels in testis and thymus, tissues which contain a large number of proliferating cells, but is not detected in most other benign tissues (PMID:1639825). It has 2 isoforms produced by alternative splicing (PMID:25137017).

实验方案

Product Specific Protocols
WB protocol for TTK antibody 84744-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols