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- KD/KO Validated
TTL Monoclonal antibody
TTL Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2E5F8
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, fetal human brain tissue, SH-SY5Y cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66076-1-Ig targets TTL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Immunogen | TTL fusion protein Ag4708 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | tubulin tyrosine ligase |
| Synonyms | TTL, Tubulin tyrosine ligase, tubulin tyrosine ligase |
| Calculated Molecular Weight | 377 aa, 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC036819 |
| Gene Symbol | TTL |
| Gene ID (NCBI) | 150465 |
| RRID | AB_11182924 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NG68 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TTL antibody 66076-1-Ig | Download protocol |
| IHC protocol for TTL antibody 66076-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Carcinogenesis Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing. | ||
Cell Rep Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.
| ||
J Cell Sci Efficient axonal transport of endolysosomes relies on the balanced ratio of microtubule tyrosination and detyrosination |
