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- KD/KO Validated
TTL Monoclonal antibody
TTL Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2E5F8
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, fetal human brain tissue, SH-SY5Y cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66076-1-Ig targets TTL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse |
Immunogen | TTL fusion protein Ag4708 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | tubulin tyrosine ligase |
Synonyms | TTL, Tubulin tyrosine ligase, tubulin tyrosine ligase |
Calculated Molecular Weight | 377 aa, 43 kDa |
Observed Molecular Weight | 43 kDa |
GenBank Accession Number | BC036819 |
Gene Symbol | TTL |
Gene ID (NCBI) | 150465 |
RRID | AB_11182924 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q8NG68 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TTL antibody 66076-1-Ig | Download protocol |
IHC protocol for TTL antibody 66076-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Carcinogenesis Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing. | ||
Cell Rep Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.
| ||
J Cell Sci Efficient axonal transport of endolysosomes relies on the balanced ratio of microtubule tyrosination and detyrosination |