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  • Featured Product
  • KD/KO Validated

TTL Monoclonal antibody, PBS Only

TTL Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E5F8

Cat no : 66076-1-PBS

Print datasheet

Synonyms

Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25, 2E5F8

验证数据展示

  • WB analysis using 66076-1-Ig (same clone as 66076-1-PBS)

    WB analysis using 66076-1-Ig (same clone as 66076-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

    IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

    IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

产品信息

66076-1-PBS targets TTL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen TTL fusion protein Ag4708 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tubulin tyrosine ligase
Synonyms Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25, 2E5F8
Calculated Molecular Weight 377 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC036819
Gene Symbol TTL
Gene ID (NCBI) 150465
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NG68
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.