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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 68044-1-Ig

    WB analysis using 68044-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68044-1-Ig (TTLL12 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68044-1-Ig

    IHC staining of human lung cancer using 68044-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68044-1-Ig (TTLL12 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTLL12 antibody included

TTLL12 Monoclonal antibody

TTLL12 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68044-1-Ig

产品说明书

CloneNo. 1H5C11

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

dJ526I14.2, FLJ41795, KIAA0153, TTLL12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inSKOV-3 cells, LNCap cells, PC-3 cells, A431 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TTLL12 Ready-To-Use IHC Kit. TTLL12 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68044-1-Ig targets TTLL12 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 TTLL12 fusion protein Ag13804 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin tyrosine ligase-like family, member 12
别名 dJ526I14.2, FLJ41795, KIAA0153, TTLL12
计算分子量 644 aa, 74 kDa
观测分子量72-74 kDa
GenBank蛋白编号BC001070
基因名称 TTLL12
Gene ID (NCBI) 23170
RRIDAB_2918786
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14166
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TTLL12 antibody 68044-1-IgDownload protocol
IHC protocol for TTLL12 antibody 68044-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols