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验证数据展示

  • FC experiment of A431 using CL488-68044

    FC experiment of A431 using CL488-68044

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TTLL12 (CL488-68044, Clone:1H5C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated TTLL12 Monoclonal antibody

TTLL12 Monoclonal Antibody for FC (Intra)
Cat No. CL488-68044

产品说明书

CloneNo. 1H5C11

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

FC (Intra)

别名

TTLL12, Tubulin--tyrosine ligase-like protein 12, 1H5C11, Inactive tubulin--tyrosine ligase-like protein 12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy TTLL12 Ready-To-Use IHC Kit. TTLL12 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68044-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68044 targets TTLL12 in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 TTLL12 fusion protein Ag13804 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin tyrosine ligase-like family, member 12
别名 TTLL12, Tubulin--tyrosine ligase-like protein 12, 1H5C11, Inactive tubulin--tyrosine ligase-like protein 12
计算分子量 644 aa, 74 kDa
观测分子量72-74 kDa
GenBank蛋白编号BC001070
基因名称 TTLL12
Gene ID (NCBI) 23170
RRIDAB_2934614
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14166
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TTLL12 antibody CL488-68044Download protocol
Standard Protocols
Click here to view our Standard Protocols