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TUFM Monoclonal antibody, PBS Only

TUFM Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A10D2

Cat no : 67802-1-PBS

Print datasheet

Synonyms

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

验证数据展示

  • WB analysis using 67802-1-Ig (same clone as 67802-1-PBS)

    WB analysis using 67802-1-Ig (same clone as 67802-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67802-1-Ig (same clone as 67802-1-PBS)

    IHC staining of human colon cancer using 67802-1-Ig (same clone as 67802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 67802-1-Ig (same clone as 67802-1-PBS)

    IP experiment of HepG2 using 67802-1-Ig (same clone as 67802-1-PBS)

    IP result of anti-TUFM (IP:67802-1-Ig, 4ug; Detection:67802-1-Ig 1:3000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

产品信息

67802-1-PBS targets TUFM in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TUFM fusion protein Ag24976 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Tu translation elongation factor, mitochondrial
Synonyms EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4
Calculated Molecular Weight 50 kDa
Observed Molecular Weight48-50 kDa
GenBank Accession NumberBC010041
Gene Symbol TUFM
Gene ID (NCBI) 7284
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49411
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.