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TUFM Monoclonal antibody

TUFM Monoclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2A10D2

Cat no : 67802-1-Ig

Print datasheet

Synonyms

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

验证数据展示

  • WB analysis using 67802-1-Ig

    WB analysis using 67802-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67802-1-Ig

    IHC staining of human colon cancer using 67802-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HepG2 using 67802-1-Ig

    IP experiment of HepG2 using 67802-1-Ig

    IP result of anti-TUFM (IP:67802-1-Ig, 4ug; Detection:67802-1-Ig 1:3000) with HepG2 cells lysate 2000 ug.

经过测试的应用

Positive WB detected inLNCaP cells, HCT 116 cells, HepG2 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, 4T1 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67802-1-Ig targets TUFM in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TUFM fusion protein Ag24976 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Tu translation elongation factor, mitochondrial
Synonyms EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4
Calculated Molecular Weight 50 kDa
Observed Molecular Weight48-50 kDa
GenBank Accession NumberBC010041
Gene Symbol TUFM
Gene ID (NCBI) 7284
RRIDAB_2918565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49411
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

实验方案

Product Specific Protocols
WB protocol for TUFM antibody 67802-1-IgDownload protocol
IHC protocol for TUFM antibody 67802-1-IgDownload protocol
IP protocol for TUFM antibody 67802-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols