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CoraLite® Plus 488-conjugated TUFM Monoclonal antibody

TUFM Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A10D2

Cat No : CL488-67802

Print datasheet

Synonyms

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

验证数据展示

  • IF Staining of HepG2 using CL488-67802

    IF Staining of HepG2 using CL488-67802

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TUFM antibody (CL488-67802, Clone: 2A10D2 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-67802

    FC experiment of HepG2 using CL488-67802

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TUFM (CL488-67802, Clone:2A10D2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67802-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67802 targets TUFM in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen TUFM fusion protein Ag24976 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Tu translation elongation factor, mitochondrial
Synonyms EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4
Calculated Molecular Weight 50 kDa
GenBank Accession NumberBC010041
Gene Symbol TUFM
Gene ID (NCBI) 7284
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49411
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TUFM antibody CL488-67802Download protocol
FC protocol for CL Plus 488 TUFM antibody CL488-67802Download protocol
Standard Protocols
Click here to view our Standard Protocols