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TULP1 Polyclonal antibody

TULP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18971-1-AP

Print datasheet

Synonyms

TUBL1, Tubby-related protein 1, Tubby-like protein 1

验证数据展示

  • WB analysis of human brain using 18971-1-AP

    WB analysis of human brain using 18971-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18971-1-AP (TULP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 18971-1-AP

    WB analysis using 18971-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18971-1-AP (TULP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human eye using 18971-1-AP

    IHC staining of human eye using 18971-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 18971-1-AP (TULP1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human eye using 18971-1-AP

    IHC staining of human eye using 18971-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 18971-1-AP (TULP1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inY79 cells, human brain tissue, mouse retina tissue
Positive IHC detected inhuman eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18971-1-AP targets TULP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen TULP1 fusion protein Ag5598 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubby like protein 1
Synonyms TUBL1, Tubby-related protein 1, Tubby-like protein 1
Calculated Molecular Weight 489 aa, 55 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC032714
Gene Symbol TULP1
Gene ID (NCBI) 7287
RRIDAB_2211412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00294
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TULP1, also known as RP14, LCA15, and TUBL1, is a member of the tubby-like protein (TULP) family. TULP1 is expressed exclusively in the retina and is essential for the transport of rhodopsin from its site of synthesis in the inner segments through the connecting cilium to the outer segments(PMID: 17620573). Mutations in TULP1 are associated with Retinitis pigmentosa 14 and Leber congenital amaurosis-15(PMID: 17620573, 17962469).

实验方案

Product Specific Protocols
WB protocol for TULP1 antibody 18971-1-APDownload protocol
IHC protocol for TULP1 antibody 18971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Rep

Proteomics identifies multiple retinitis pigmentosa associated proteins involved in retinal degeneration in a mouse model bearing a Pde6b mutation

Authors - Mingzhu Yang
View Article