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验证数据展示

  • WB analysis using 68335-1-Ig

    WB analysis using 68335-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68335-1-Ig (TXNDC9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of HEK-293 using 68335-1-Ig

    WB analysis of HEK-293 using 68335-1-Ig

    WB result of TXNDC9 antibody (68335-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC9 transfected HEK-293 cells.

  • FC experiment of HEK-293 using 68335-1-Ig

    FC experiment of HEK-293 using 68335-1-Ig

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug TXNDC9 Monoclonal antibody (68335-1-Ig, Clone:2A10G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO验证

TXNDC9 Monoclonal antibody

TXNDC9 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68335-1-Ig

产品说明书

CloneNo. 2A10G3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

Thioredoxin domain-containing protein 9, Protein 1-4, Protein 1 4, PHLP3, ATP-binding protein associated with cell differentiation

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HEK-293 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68335-1-Ig targets TXNDC9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 TXNDC9 fusion protein Ag33259 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 thioredoxin domain containing 9
别名 Thioredoxin domain-containing protein 9, Protein 1-4, Protein 1 4, PHLP3, ATP-binding protein associated with cell differentiation
计算分子量 226 aa, 27 kDa
观测分子量27 kDa
GenBank蛋白编号BC005968
基因名称 TXNDC9
Gene ID (NCBI) 10190
RRIDAB_3085059
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO14530
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXNDC9, also known as APACD, has a negative effect on protein folding by significantly reducing the activity of chaperone protein TCP1 complex ATPase activity. Including actin or tubulin.

实验方案

Product Specific Protocols
WB protocol for TXNDC9 antibody 68335-1-IgDownload protocol
FC protocol for TXNDC9 antibody 68335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols