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验证数据展示

  • WB analysis using 66725-1-Ig (same clone as 66725-1-PBS)

    WB analysis using 66725-1-Ig (same clone as 66725-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66725-1-Ig (TYMS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66725-1-Ig (same clone as 66725-1-PBS)

    WB analysis of HEK-293 using 66725-1-Ig (same clone as 66725-1-PBS)

    WB result of TYMS antibody (66725-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TYMS transfected HEK-293 cells. This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    IHC staining of human colon cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TYMS antibody included
  • IHC staining of human colon cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    IHC staining of human colon cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TYMS antibody included
  • IHC staining of human pancreas cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    IHC staining of human pancreas cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:400 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TYMS antibody included
  • IHC staining of human pancreas cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    IHC staining of human pancreas cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:400 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TYMS antibody included
  • IF Staining of HeLa using 66725-1-Ig (same clone as 66725-1-PBS)

    IF Staining of HeLa using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TYMS antibody (66725-1-Ig, Clone: 1F5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66725-1-Ig (same clone as 66725-1-PBS)

    FC experiment of HepG2 using 66725-1-Ig (same clone as 66725-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TYMS Monoclonal antibody (66725-1-Ig, Clone:1F5B7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TYMS Monoclonal antibody, PBS Only

TYMS Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66725-1-PBS

产品说明书

CloneNo. 1F5B7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Thymidylate synthase, OK/SW-cl.29, HsT422, EC:2.1.1.45, DKCD

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TYMS Ready-To-Use IHC Kit. TYMS primary antibody included.

产品信息

66725-1-PBS targets TYMS in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 TYMS fusion protein Ag7027 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 thymidylate synthetase
别名 Thymidylate synthase, OK/SW-cl.29, HsT422, EC:2.1.1.45, DKCD
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC002567
基因名称 TYMS
Gene ID (NCBI) 7298
RRIDAB_2882076
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04818
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.