验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-66725 targets TYMS in WB applications and shows reactivity with Human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human |
| 免疫原 | TYMS fusion protein Ag7027 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | thymidylate synthetase |
| 别名 | HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS |
| 计算分子量 | 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC002567 |
| 基因名称 | TYMS |
| Gene ID (NCBI) | 7298 |
| RRID | AB_2883845 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P04818 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP TYMS antibody HRP-66725 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |