HRP-conjugated TYMS Monoclonal antibody
TYMS Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB
Conjugate
HRP
CloneNo.
1F5B7
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy TYMS Ready-To-Use IHC Kit. TYMS primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:20000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
HRP-66725 targets TYMS in WB applications and shows reactivity with Human samples.
Tested Applications | WB Application Description |
Tested Reactivity | Human |
Immunogen | TYMS fusion protein Ag7027 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | thymidylate synthetase |
Synonyms | HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 36 kDa |
GenBank Accession Number | BC002567 |
Gene Symbol | TYMS |
Gene ID (NCBI) | 7298 |
RRID | AB_2883845 |
Conjugate | HRP |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P04818 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.
实验方案
Product Specific Protocols | |
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WB protocol for HRP TYMS antibody HRP-66725 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |