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CD141/Thrombomodulin Recombinant antibody

CD141/Thrombomodulin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240365D1

Cat no : 83416-4-RR

Print datasheet

Synonyms

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

验证数据展示

  • WB analysis using 83416-4-RR

    WB analysis using 83416-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83416-4-RR (Thrombomodulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 83416-4-RR

    IHC staining of human lung using 83416-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung using 83416-4-RR

    IHC staining of human lung using 83416-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 83416-4-RR

    IF Staining of A431 using 83416-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Thrombomodulin antibody (83416-4-RR, Clone: 240365D1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83416-4-RR

    Affinity and Kinetic Characterization of 83416-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83416-4-RR against Human CD141/Thrombomodulin were performed. The affinity constant is 1.15 nM.

经过测试的应用

Positive WB detected inTHP-1 cells, A431 cells
Positive IHC detected inhuman lung tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83416-4-RR is CD141/Thrombomodulin Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name thrombomodulin
Synonyms THBD, Thrombomodulin, THRM, Fetomodulin, CD141
Calculated Molecular Weight60 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberNM_000361.3
Gene Symbol THBD
Gene ID (NCBI) 7056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP07204
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

实验方案

Product Specific Protocols
WB protocol for CD141/Thrombomodulin antibody 83416-4-RRDownload protocol
IHC protocol for CD141/Thrombomodulin antibody 83416-4-RRDownload protocol
IF protocol for CD141/Thrombomodulin antibody 83416-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols