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验证数据展示

  • WB analysis using 67535-1-Ig (same clone as 67535-1-PBS)

    WB analysis using 67535-1-Ig (same clone as 67535-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67535-1-Ig (Tim23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

    IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

    IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67535-1-Ig (same clone as 67535-1-PBS)

    IF Staining of HepG2 using 67535-1-Ig (same clone as 67535-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tim23 antibody (67535-1-Ig, Clone: 3H10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

Tim23 Monoclonal antibody, PBS Only

Tim23 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67535-1-PBS

产品说明书

CloneNo. 3H10F5

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PRO1197, TIM23, TIMM23

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67535-1-PBS targets Tim23 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 Tim23 fusion protein Ag27069 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 translocase of inner mitochondrial membrane 23
别名 PRO1197, TIM23, TIMM23
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC062707
基因名称 TIMM23
Gene ID (NCBI) 100287932
RRIDAB_2882754
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO14925
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.