验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, RAW 264.7 cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IF | See 1 publications below |
产品信息
67535-1-Ig targets Tim23 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Immunogen | Tim23 fusion protein Ag27069 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | translocase of inner mitochondrial membrane 23 homolog (yeast) |
| Synonyms | PRO1197, TIM23, TIMM23 |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC062707 |
| Gene Symbol | Tim23 |
| Gene ID (NCBI) | 10431 |
| RRID | AB_2882754 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O14925 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Tim23 antibody 67535-1-Ig | Download protocol |
| IHC protocol for Tim23 antibody 67535-1-Ig | Download protocol |
| IF protocol for Tim23 antibody 67535-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy The nucleoprotein of influenza A virus inhibits the innate immune response by inducing mitophagy | ||
Autophagy Mycobacterium bovis induces mitophagy to suppress host xenophagy for its intracellular survival. | ||
Mol Med 4-Octyl itaconate attenuates LPS-induced acute kidney injury by activating Nrf2 and inhibiting STAT3 signaling | ||
Zool Res Choline dehydrogenase interacts with SQSTM1 to activate mitophagy and promote coelomocyte survival in Apostichopus japonicus following Vibrio splendidus infection | ||
Front Pharmacol Low-dose of caffeine alleviates high altitude pulmonary edema via regulating mitochondrial quality control process in AT1 cells | ||
Commun Biol RABIF promotes hepatocellular carcinoma progression through regulation of mitophagy and glycolysis |