验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 67535-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67535 targets Tim23 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | Tim23 fusion protein Ag27069 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | translocase of inner mitochondrial membrane 23 homolog (yeast) |
| Synonyms | PRO1197, TIM23, TIMM23 |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC062707 |
| Gene Symbol | Tim23 |
| Gene ID (NCBI) | 10431 |
| RRID | AB_2919495 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O14925 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Tim23 antibody CL488-67535 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |