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  • Featured Product
  • KD/KO Validated

Transgelin 2 Monoclonal antibody, PBS Only

Transgelin 2 Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2E5G1

Cat No : 60044-1-PBS

Print datasheet

Synonyms

SM22 alpha homolog, SM22 beta, SM22beta, TAGLN2, Transgelin 2, Transgelin 2/TAGLN2

验证数据展示

  • WB analysis of K-562 using 60044-1-Ig (same clone as 60044-1-PBS)

    WB analysis of K-562 using 60044-1-Ig (same clone as 60044-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 60044-1-Ig (same clone as 60044-1-PBS)

    IHC staining of human urothelial carcinoma using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 60044-1-Ig (same clone as 60044-1-PBS)

    IP experiment of HEK-293 using 60044-1-Ig (same clone as 60044-1-PBS)

    IP result of anti-Transgelin 2 (IP:60044-1-Ig, 4ug; Detection:60044-1-Ig 1:1000) with HEK-293 cells lysate 1000ug. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60044-1-Ig (same clone as 60044-1-PBS)

    IF Staining of HepG2 using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

产品信息

60044-1-PBS targets Transgelin 2 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse
Immunogen Transgelin 2 fusion protein Ag0337 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transgelin 2
Synonyms SM22 alpha homolog, SM22 beta, SM22beta, TAGLN2, Transgelin 2, Transgelin 2/TAGLN2
Calculated Molecular Weight 199 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC002616
Gene Symbol TAGLN2
Gene ID (NCBI) 8407
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP37802
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.