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验证数据展示

  • WB analysis of K-562 using 60044-1-Ig (same clone as 60044-1-PBS)

    WB analysis of K-562 using 60044-1-Ig (same clone as 60044-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 60044-1-Ig (same clone as 60044-1-PBS)

    IHC staining of human urothelial carcinoma using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 60044-1-Ig (same clone as 60044-1-PBS)

    IP experiment of HEK-293 using 60044-1-Ig (same clone as 60044-1-PBS)

    IP result of anti-Transgelin 2 (IP:60044-1-Ig, 4ug; Detection:60044-1-Ig 1:1000) with HEK-293 cells lysate 1000ug. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 60044-1-Ig (same clone as 60044-1-PBS)

    WB analysis of MCF-7 using 60044-1-Ig (same clone as 60044-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60044-1-Ig (same clone as 60044-1-PBS)

    IF Staining of HepG2 using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Transgelin 2 Monoclonal antibody, PBS Only

Transgelin 2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 60044-1-PBS

产品说明书

CloneNo. 2E5G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

别名

TAGLN2, 2E5G1, CDABP0035, Epididymis tissue protein Li 7e, SM22-alpha homolog

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60044-1-PBS targets Transgelin 2 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Transgelin 2 fusion protein Ag0337 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transgelin 2
别名 TAGLN2, 2E5G1, CDABP0035, Epididymis tissue protein Li 7e, SM22-alpha homolog
计算分子量 199 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC002616
基因名称 Transgelin-2/TAGLN2
Gene ID (NCBI) 8407
RRIDAB_2271433
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP37802
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.