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Transgelin 2 Monoclonal antibody
Transgelin 2 Monoclonal Antibody for IF/ICC,IF-P, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC,IF-P, IHC, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2E5G1
验证数据展示
经过测试的应用
Positive WB detected in | K562 cells, K-562 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human urothelial carcinoma tissue, human colon tissue, human colon cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human colon cancer tissue, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60044-1-Ig targets Transgelin 2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC,IF-P, IHC, IP, WB, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | Transgelin 2 fusion protein Ag0337 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | transgelin 2 |
Synonyms | SM22 alpha homolog, SM22 beta, SM22beta, TAGLN2, Transgelin 2, Transgelin 2/TAGLN2 |
Calculated Molecular Weight | 199 aa, 22 kDa |
Observed Molecular Weight | 22 kDa |
GenBank Accession Number | BC002616 |
Gene Symbol | TAGLN2 |
Gene ID (NCBI) | 8407 |
RRID | AB_2271433 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P37802 |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Transgelin 2 antibody 60044-1-Ig | Download protocol |
IHC protocol for Transgelin 2 antibody 60044-1-Ig | Download protocol |
IF protocol for Transgelin 2 antibody 60044-1-Ig | Download protocol |
IP protocol for Transgelin 2 antibody 60044-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Med Rep Downregulation of transgelin 2 promotes breast cancer metastasis by activating the reactive oxygen species/nuclear factor‑κB signaling pathway.
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Nat Commun Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer's disease | ||
Cell Signal TAGLN2 targeted control of ARPC5-mediated activation of the MEK/ERK signaling pathway influences the proliferation, invasion, and metastasis of pancreatic cancer cells |