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验证数据展示

  • IF Staining of HepG2 using CL488-60044

    IF Staining of HepG2 using CL488-60044

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Transgelin 2 antibody (CL488-60044, Clone: 2E5G1 ) at dilution of 1:200.

  • IF Staining of human colon cancer using CL488-60044

    IF Staining of human colon cancer using CL488-60044

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 Transgelin 2 antibody (CL488-60044, Clone: 2E5G1 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Transgelin 2 Monoclonal antibody

Transgelin 2 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-60044

产品说明书

CloneNo. 2E5G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, IF-P

别名

TAGLN2, Transgelin 2/TAGLN2, SM22-alpha homolog, Epididymis tissue protein Li 7e, CDABP0035

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60044-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60044 targets Transgelin 2 in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse
免疫原 Transgelin 2 fusion protein Ag0337 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transgelin 2
别名 TAGLN2, Transgelin 2/TAGLN2, SM22-alpha homolog, Epididymis tissue protein Li 7e, CDABP0035
计算分子量 199 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC002616
基因名称 Transgelin-2/TAGLN2
Gene ID (NCBI) 8407
RRIDAB_3084132
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP37802
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Transgelin 2 antibody CL488-60044Download protocol
Standard Protocols
Click here to view our Standard Protocols