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验证数据展示

  • FC experiment of HepG2 using CL647-15508

    FC experiment of HepG2 using CL647-15508

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Transgelin-2-specific (CL647-15508) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL647-15508

    IF Staining of HepG2 using CL647-15508

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 Transgelin-2-specific antibody (CL647-15508) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Transgelin-2-specific Polyclonal antibody

Transgelin-2-specific Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-15508

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

Transgelin 2, TAGLN2, Transgelin 2/TAGLN2, SM22-alpha homolog, Epididymis tissue protein Li 7e

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 15508-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-15508 targets Transgelin-2-specific in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transgelin 2
Synonyms Transgelin 2, TAGLN2, Transgelin 2/TAGLN2, SM22-alpha homolog, Epididymis tissue protein Li 7e
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberNM_003564
Gene Symbol Transgelin-2/TAGLN2
Gene ID (NCBI) 8407
RRIDAB_2934916
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP37802
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody can bind transgelin 2 specifically, but not trangelin 1 or 3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Transgelin-2-specific antibody CL647-15508Download protocol
FC protocol for CL Plus 647 Transgelin-2-specific antibody CL647-15508Download protocol
Standard Protocols
Click here to view our Standard Protocols