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验证数据展示

  • WB analysis using 66016-1-Ig (same clone as 66016-1-PBS)

    WB analysis using 66016-1-Ig (same clone as 66016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

    IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TKT antibody included
  • IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

    IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TKT antibody included
  • IF Staining of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

    IF Staining of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transketolase antibody (66016-1-Ig, Clone: 3E2F3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

Transketolase Monoclonal antibody, PBS Only

Transketolase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66016-1-PBS

产品说明书

CloneNo. 3E2F3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

TKT, TK, EC:2.2.1.1, 3E2F3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TKT Ready-To-Use IHC Kit. TKT primary antibody included.

产品信息

66016-1-PBS targets Transketolase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Transketolase fusion protein Ag16779 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 transketolase
别名 TKT, TK, EC:2.2.1.1, 3E2F3
计算分子量 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC009970
基因名称 Transketolase
Gene ID (NCBI) 7086
RRIDAB_11064729
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP29401
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.