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Transketolase Monoclonal antibody, PBS Only

Transketolase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E2F3

Cat no : 66016-1-PBS

Print datasheet

Synonyms

TKT, TK, EC:2.2.1.1, 3E2F3

验证数据展示

  • WB analysis using 66016-1-Ig (same clone as 66016-1-PBS)

    WB analysis using 66016-1-Ig (same clone as 66016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

    IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TKT antibody included
  • IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

    IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TKT antibody included
  • IF Staining of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

    IF Staining of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transketolase antibody (66016-1-Ig, Clone: 3E2F3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TKT Ready-To-Use IHC Kit. TKT primary antibody included.

产品信息

66016-1-PBS targets Transketolase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Transketolase fusion protein Ag16779 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transketolase
Synonyms TKT, TK, EC:2.2.1.1, 3E2F3
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC009970
Gene Symbol Transketolase
Gene ID (NCBI) 7086
RRIDAB_11064729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29401
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.