验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, human liver tissue, Jurkat cells, human brain tissue, HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy TKT Ready-To-Use IHC Kit. TKT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
66016-1-Ig targets Transketolase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Transketolase fusion protein Ag16779 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transketolase |
| 别名 | TKT, TK, EC:2.2.1.1, 3E2F3 |
| 计算分子量 | 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC009970 |
| 基因名称 | Transketolase |
| Gene ID (NCBI) | 7086 |
| RRID | AB_11064729 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P29401 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Transketolase antibody 66016-1-Ig | Download protocol |
| IHC protocol for Transketolase antibody 66016-1-Ig | Download protocol |
| IF protocol for Transketolase antibody 66016-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Med Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma | ||
FASEB J Prolactin receptor potentiates chemotherapy through miRNAs-induced G6PD/TKT inhibition in pancreatic cancer |
