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CoraLite® Plus 488-conjugated Transketolase Monoclonal antibody

Transketolase Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E2F3

Cat no : CL488-66016

Print datasheet

Synonyms

TKT, TK, EC:2.2.1.1, 3E2F3

验证数据展示

  • IF Staining of HepG2 using CL488-66016

    IF Staining of HepG2 using CL488-66016

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Transketolase antibody (CL488-66016, Clone: 3E2F3 ) at dilution of 1:600, CL594-phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TKT Ready-To-Use IHC Kit. TKT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66016-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66016 targets Transketolase in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Transketolase fusion protein Ag16779 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transketolase
Synonyms TKT, TK, EC:2.2.1.1, 3E2F3
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC009970
Gene Symbol Transketolase
Gene ID (NCBI) 7086
RRIDAB_2883228
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29401
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Transketolase antibody CL488-66016Download protocol
Standard Protocols
Click here to view our Standard Protocols