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TrpRS Monoclonal antibody, PBS Only

TrpRS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C6C3

Cat no : 67952-1-PBS

Print datasheet

Synonyms

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

验证数据展示

  • WB analysis using 67952-1-Ig (same clone as 67952-1-PBS)

    WB analysis using 67952-1-Ig (same clone as 67952-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

  • IF Staining of LNCaP using 67952-1-Ig (same clone as 67952-1-PBS)

    IF Staining of LNCaP using 67952-1-Ig (same clone as 67952-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using TrpRS antibody (67952-1-Ig, Clone: 1C6C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy WARS Ready-To-Use IHC Kit. WARS primary antibody included.

产品信息

67952-1-PBS targets TrpRS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen TrpRS fusion protein Ag31430 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tryptophanyl-tRNA synthetase
Synonyms GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS
Calculated Molecular Weight 471 aa, 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC017489
Gene Symbol WARS1
Gene ID (NCBI) 7453
RRIDAB_2918704
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23381
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).