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验证数据展示

  • WB analysis using 67952-1-Ig

    WB analysis using 67952-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of LNCaP using 67952-1-Ig

    IF Staining of LNCaP using 67952-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using TrpRS antibody (67952-1-Ig, Clone: 1C6C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

TrpRS Monoclonal antibody

TrpRS Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67952-1-Ig

产品说明书

CloneNo. 1C6C3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat, mouse

应用

WB, IF/ICC, ELISA

别名

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, 4T1 cells, human placenta tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inLNCaP cells
Planning an IHC experiment? We recommend our IHCeasy WARS Ready-To-Use IHC Kit. WARS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67952-1-Ig targets TrpRS in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原 TrpRS fusion protein Ag31430 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tryptophanyl-tRNA synthetase
别名 GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS
计算分子量 471 aa, 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC017489
基因名称 WARS1
Gene ID (NCBI) 7453
RRIDAB_2918704
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23381
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).

实验方案

Product Specific Protocols
WB protocol for TrpRS antibody 67952-1-IgDownload protocol
IF protocol for TrpRS antibody 67952-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols