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验证数据展示

  • IF Staining of MCF-7 using CL488-67952

    IF Staining of MCF-7 using CL488-67952

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 TrpRS antibody (CL488-67952, Clone: 1C6C3 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67952

    FC experiment of HeLa using CL488-67952

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TrpRS (CL488-67952, Clone:1C6C3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated TrpRS Monoclonal antibody

TrpRS Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67952

产品说明书

CloneNo. 1C6C3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat, mouse

应用

IF/ICC, FC (Intra)

别名

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy WARS Ready-To-Use IHC Kit. WARS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67952-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67952 targets TrpRS in IF/ICC, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human, rat, mouse
免疫原 TrpRS fusion protein Ag31430 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tryptophanyl-tRNA synthetase
别名 GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS
计算分子量 471 aa, 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC017489
基因名称 WARS1
Gene ID (NCBI) 7453
RRIDAB_2934596
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23381
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TrpRS antibody CL488-67952Download protocol
FC protocol for CL Plus 488 TrpRS antibody CL488-67952Download protocol
Standard Protocols
Click here to view our Standard Protocols