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验证数据展示

  • IF Staining of HEK-293 using CL488-68166

    IF Staining of HEK-293 using CL488-68166

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 U2AF2 antibody (CL488-68166, Clone: 1B7E7 ) at dilution of 1:100, CL594-phalloidin (red).

  • FC experiment of HEK-293 using CL488-68166

    FC experiment of HEK-293 using CL488-68166

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human U2AF2 (CL488-68166, Clone:1B7E7) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated U2AF2/U2AF65 Monoclonal antibody

U2AF2/U2AF65 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68166

产品说明书

CloneNo. 1B7E7

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

U2AF65, U2AF2, hU2AF65, hU2AF(65), 1B7E7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy U2AF2 Ready-To-Use IHC Kit. U2AF2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68166-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68166 targets U2AF2/U2AF65 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 U2AF2/U2AF65 fusion protein Ag8070 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 U2 small nuclear RNA auxiliary factor 2
别名 U2AF65, U2AF2, hU2AF65, hU2AF(65), 1B7E7
计算分子量 54 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC008740
基因名称 U2AF2
Gene ID (NCBI) 11338
RRIDAB_3084427
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP26368
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 U2AF2/U2AF65 antibody CL488-68166Download protocol
FC protocol for CL Plus 488 U2AF2/U2AF65 antibody CL488-68166Download protocol
Standard Protocols
Click here to view our Standard Protocols