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验证数据展示

  • IF Staining of PC-3 using CL594-67545

    IF Staining of PC-3 using CL594-67545

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite®594 UAP1 antibody (CL594-67545, Clone: 2C7B2 ) at dilution of 1:200.

  • FC experiment of PC-3 using CL594-67545

    FC experiment of PC-3 using CL594-67545

    1x10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated UAP1 Monoclonal antibody (CL594-67545, Clone:2C7B2)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated UAP1 Monoclonal antibody

UAP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67545

Print datasheet

CloneNo. 2C7B2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

AntigenX, Antigen X, AGX1, AGX 2, AgX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 67545-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67545 targets UAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen UAP1 fusion protein Ag9513 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name UDP-N-acteylglucosamine pyrophosphorylase 1
Synonyms AntigenX, Antigen X, AGX1, AGX 2, AgX
Calculated Molecular Weight 505 aa, 57 kDa
Observed Molecular Weight 57-59 kDa
GenBank Accession NumberBC009377
Gene Symbol UAP1
Gene ID (NCBI) 6675
RRIDAB_3084775
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16222
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)

实验方案

Product Specific Protocols
IF protocol for CL594 UAP1 antibody CL594-67545Download protocol
FC protocol for CL594 UAP1 antibody CL594-67545Download protocol
Standard Protocols
Click here to view our Standard Protocols