验证数据展示
经过测试的应用
Positive IF/ICC detected in | PC-3 cells |
Positive FC (Intra) detected in | PC-3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-67545 targets UAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human |
免疫原 | UAP1 fusion protein Ag9513 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | UDP-N-acteylglucosamine pyrophosphorylase 1 |
别名 | AntigenX, Antigen X, AGX1, AGX 2, AgX |
计算分子量 | 505 aa, 57 kDa |
观测分子量 | 57-59 kDa |
GenBank蛋白编号 | BC009377 |
基因名称 | UAP1 |
Gene ID (NCBI) | 6675 |
RRID | AB_3084775 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q16222 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)
实验方案
Product Specific Protocols | |
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IF protocol for CL594 UAP1 antibody CL594-67545 | Download protocol |
FC protocol for CL594 UAP1 antibody CL594-67545 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |