验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, mouse brain tissue, rabbit brain tissue, rat brain tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
67385-1-Ig targets UBAC1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, CoIP |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human |
| Immunogen | UBAC1 fusion protein Ag13958 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | UBA domain containing 1 |
| Synonyms | KPC2, UBA domain containing 1, Kip1 ubiquitination-promoting complex protein 2, Glialblastoma cell differentiation-related protein 1, GBDR1 |
| Calculated Molecular Weight | 405 aa, 45 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC004967 |
| Gene Symbol | UBAC1 |
| Gene ID (NCBI) | 10422 |
| RRID | AB_2882630 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9BSL1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBAC1 antibody 67385-1-Ig | Download protocol |
| IF protocol for UBAC1 antibody 67385-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |