验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, mouse brain tissue, rabbit brain tissue, rat brain tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
67385-1-Ig targets UBAC1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 文献引用反应性 | human |
| 免疫原 | UBAC1 fusion protein Ag13958 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | UBA domain containing 1 |
| 别名 | KPC2, UBA domain containing 1, Kip1 ubiquitination-promoting complex protein 2, Glialblastoma cell differentiation-related protein 1, GBDR1 |
| 计算分子量 | 405 aa, 45 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC004967 |
| 基因名称 | UBAC1 |
| Gene ID (NCBI) | 10422 |
| RRID | AB_2882630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9BSL1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UBAC1 antibody 67385-1-Ig | Download protocol |
| IF protocol for UBAC1 antibody 67385-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |