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UBAC1 Monoclonal antibody, PBS Only

UBAC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A3G7

Cat no : 67385-1-PBS

Print datasheet

Synonyms

KPC2, UBA domain containing 1, Kip1 ubiquitination-promoting complex protein 2, Glialblastoma cell differentiation-related protein 1, GBDR1

验证数据展示

  • WB analysis using 67385-1-Ig (same clone as 67385-1-PBS)

    WB analysis using 67385-1-Ig (same clone as 67385-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67385-1-Ig (UBAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67385-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67385-1-Ig (same clone as 67385-1-PBS)

    IF Staining of HeLa using 67385-1-Ig (same clone as 67385-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using UBAC1 antibody (67385-1-Ig, Clone: 2A3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67385-1-PBS in a different storage buffer formulation.

产品信息

67385-1-PBS targets UBAC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen UBAC1 fusion protein Ag13958 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name UBA domain containing 1
Synonyms KPC2, UBA domain containing 1, Kip1 ubiquitination-promoting complex protein 2, Glialblastoma cell differentiation-related protein 1, GBDR1
Calculated Molecular Weight 405 aa, 45 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC004967
Gene Symbol UBAC1
Gene ID (NCBI) 10422
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BSL1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung.