UBC9 Monoclonal antibody
UBC9 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
4A10B11
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, rat kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 1 publications below |
产品信息
The immunogen of 60201-1-Ig is UBC9 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, monkey |
Immunogen | UBC9 fusion protein Ag7885 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) |
Synonyms | C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I |
Calculated Molecular Weight | 18 kDa |
Observed Molecular Weight | 18 kDa |
GenBank Accession Number | BC004437 |
Gene Symbol | UBC9 |
Gene ID (NCBI) | 7329 |
RRID | AB_10918977 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P63279 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for UBC9 antibody 60201-1-Ig | Download protocol |
IHC protocol for UBC9 antibody 60201-1-Ig | Download protocol |
FC protocol for UBC9 antibody 60201-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Viruses The Interaction between SARS-CoV-2 Nucleocapsid Protein and UBC9 Inhibits MAVS Ubiquitination by Enhancing Its SUMOylation |